1st appearance of Emily Preston, an agent of S.H.I.E.L.D. who worked alongside Deadpool

1st appearance of Scott Adsit, an agent of S.H.I.E.L.D.

1st appearance of Michael Hawthorne, a necromancer

Written by comedian Brian Posehn with Gerry Duggan

1st appearance of Buck Lime, an ex-S.H.I.E.L.D. agent who partnered with Moon Knight for a short time

1st appearance of John Garrett, an agent of S.H.I.E.L.D.

1st appearance of Melinda May, an agent of S.H.I.E.L.D.

1st appearance of Leo Fitz, , an agent of S.H.I.E.L.D.

1st appearance of Jemma Simmons, an agent of S.H.I.E.L.D.

1st appearance of Contessa Valentina Allegro De Fontaine, the romantic interest of Nick Fury and a high-ranking member of S.H.I.E.L.D.

[September 2022 - confirmed for the Thunderbolts movie]

1st appearance of Gaffer, a fighter pilot

1st cameo appearance of Jacob Fury, the younger brother of Nick Fury who later becomes Scorpio then Kraken

1st appearance of Agent 13, Sharon Carter

1st cameo appearance of Peggy Carter, one panel, obscured in the distance

1st appearance of Batroc the Leaper

1st appearance of Jasper Sitwell, an agent of S.H.I.E.L.D. and top level interrogator

1st official team appearance of the New Avengers: Captain America, Spider-Man, Iron Man, Luke Cage, Spider-Woman

1st appearance of Maria Hill, once served as director of S.H.I.E.L.D.

[December 2021 - Colby Smulders to reprise her role as Maria Hill in Secret Invasion]

1st appearance of Tyrannosaurus, a symbiote impersonating Rex Strickland

1st appearance of the Grendel Symbiote

Origin of the Sym-Soldier Program aka Weapon V, a Vietnam-era initiative to bond symbiote biomass, extracted from the Grendel dragon to wounded soldiers

1st appearance of Marcus Johnson, later revealed to be Nick Fury Jr., the son of Nick Fury

1st appearance of Agent Phil Coulson in Marvel Comics continuity, referred to only by the name ‘Cheese’

1st appearance of Sgt. Nick Fury

1st team appearance of the Howling Commandos: Happy Sam Sawyer, Dino Manelli, Dum Dum Dugan, Junior Juniper, Rebel Ralston and Gabe Jones, the first ongoing black character in the mainstream Marvel Universe (post Fantastic Four #1)

In the book, Stan Lee's Amazing Marvel Universe, Stan explains Gabe Jones was intended to be black but due to a printing error, he was the same color as the other soldiers. Gabe's skin tone is noticeably different in the next issue though not necessarily a natural color

December 2020 - Fury confirmed to lead his own series alongside Talos in Secret Invasion]

Marcus Johnson revealed to be Nick Fury Jr

1st time the name Phil Coulson is used in regular Marvel Comics continuity

1st appearance of Victoria Hand, an agent of S.H.I.E.L.D. who also worked for Norman Osborn's H.A.M.M.E.R.

1st appearance of Roz Solomon, Agent of S.H.I.E.L.D.

Jane Foster reveals she has cancer

1st team appearance of S.H.I.E.L.D

1st team appearance of Hydra

Nick Fury becomes an Agent of S.H.I.E.L.D.