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Symbiotes
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- 1st appearance and origin of Lee Price, bonds with the Venom symbiote
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- Origin of Gorr the God Butcher
- 1st cameo appearance of Knull the Symbiote God
- [July 2020 - in the panels where Knull made his first cameo, he is fighting against a character in golden armor. The identity of this character is unknown but there is speculation that the golden-armored god might be developed further or appear soon]
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- 1st appearance of Gwen Stacy as Gwenom
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- Preview appearance of Bile, a cannibalistic madman with the strength and eating habits of a spider
- *Bile was presented as a joke in this issue but CBR posts an article about Donny Cates taking an interest in the character even though it seemed the tweet exchange was more conversational without future plans
- *character never appeared in a story but bears similar horror attributes as Spiders-Man of Earth 11580 (See Spider-Verse button)
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- Hulk bonds with the Venom symbiote
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- 1st appearance of Sleeper, the result of Eddie Brock bonding with the infant Symbiote
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- 1st appearance of Patricia Robertson as Scream, formerly She-Venom
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- 1st appearance of Spider-Man's black costume
- Cover swipe of Amazing Fantasy #15
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- 1st full appearance of Normie Osborn as Goblin Childe
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- 1st appearance of N'Jadaka, the new Emperor bonded to a symbiote
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- 1st team appearance of The Mercury Team, a US task force deployed to hunt down symbiotes equipped with the Life Foundation symbiotes: Phage, Lasher, Agony, Riot
- *copies ordered by retailers: 30,378
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- 1st appearance of Gorr, the God Butcher
- 1st appearance of the Necrosword, the first symbiote created by Knull