Batman and Robin  (2009)

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1st appearance of Professor Pyg, a deranged surgeon that makes people into Dollotrons
Pyg briefly appeared, albeit deceased, in Batman #666 but did not resemble the version of the character seen in this issue
[October 2022 - Matt Reeves expressed interest in developing a Professor Pyg film]
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1st cameo appearance of Flamingo
A character named Flamingo was briefly introduced, previously in Batman #666 which was a future tale featuring Damian Wayne as Batman
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1st full appearance of Flamingo, a flamboyantly dressed assassin who dabbles in cannibalism
Cover art inspired by Purple Rain by Prince and the Revolution
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Establishment of Batman Incorporated, a global crime-fighting initiative launched by Bruce Wayne
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1st appearance of the Absence, a former girlfriend of Bruce Wayne who survived a bullet to the head

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