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- 1st appearance of Monica Chang, as the Black Widow, first appeared in the mainstream Marvel Universe in Avengers A.I. #1 (2013)
- 1st appearance of Swarm as Red Wasp
- Reintroduction of characters teased to exist in comics from decades ago but redesigned with new identities: Rapture, Reject, Ruin, Wrath
- Jack of Hearts plays a prominent role