1st full appearance of Knull,the creator of the Symbiote alien race

Knull's first cameo appearance is in Thor: God of Thunder #6

The spiral emblem associated with Knull is a reference to Chthulhu mythology

Knull was the first being that existed in nothingness that preceded the creation of the current Marvel Universe

1st appearance of the Hood, Parker Robbins

[February 2022 - rumored for Ironheart, formerly rumored for Hawkeye / August 2022. - confirmed for Ironheart]

1st appearance of John King, the cousin of Parker Robbins

1st team appearance of the X-Men: Cyclops, Jean Grey aka Marvel Girl, Iceman, Angel, Beast, Professor X

1st appearance of Magneto

Homo Superior concept, coined by Magneto to describe humans born with special abilities introduced in this issue

1st appearance and origin of Captain America

1st appearance of James Buchanan Barnes aka Bucky, later becomes the Winter Soldier

1st appearance of the Red Skull, George Maxon, an agent of the true Red Skull

1st appearance of Doctor Reinstein aka Doctor Erskine, the inventor of the Super Soldier serum

1st appearance of Hurricane, later becomes Makkari

1st appearance of Tuk the Cave Boy, first offspring of the Inhumans

Introduction of Project Rebirth, the Super Soldier Program that eventually expanded into Weapon Plus

💰 In April 2022, a 9.4 sold for $3,120,000 (San Francisco Pedigree)

3rd appearance of Spider-Man

1st appearance of Vulture

1st appearance of the Terrible Tinkerer

1st cameo appearance of Mysterio, disguised as the assistant of the Terrible Tinkerer

Spider-Man first references his ability to sense danger as his Spider-Sense tingling

[June 2022 - Jorma Taccone to voice Vulture in Spider-Verse 2]

Death of Thanos

1st appearance of Lord Order and Master Chaos

1st appearance of Mephisto

1st appearance, cover and origin of Dr. Octopus

Debut of the 'Spider's Web' fan letters section

An editing error has Doctor Octopus mistakenly refer to Spider-Man as Super-Man

1st appearance of the Blob, an invulnerable mutant

1st appearance of Omega Red, a Russian serial killer, captured and experimented on by the government

1st appearance and origin of Sandman

1st appearance of Betty Brant, a secretary at the Daily Bugle

Spider-Man refers to himself as 'The Friendly Neighborhood Spider-Man' for the first time

1st cameo appearance of Jessica Jones, a spectator of the Sandman versus Spider-Man fight - this appearance is retconned as revealed in The Amazing Spider-Man #601

1st appearance and origin of Doctor Doom

[November 2020 - speculation that Doom will be hinted at or appear in the Black Panther sequel as well as other MCU projects]

1st appearance of Annihilus, ruler of the Negative Zone

1st appearance of Franklin Richards, as an unnamed infant, considered by Marvel to be the most powerful mutant in the Marvel Universe

Introduction of the Cosmic Control Rod, a weapon used by Annihilus that manipulates matter

1st full appearance and cover of Apocalypse

Apocalypse is the featured villain in Marvel's Dark Ages limited series September 2021

1st full appearance of Bucky Barnes as the Winter Soldier

[September 2022 - confirmed for the Thunderbolts movie]

1st appearance of Kang, the original version

[December 2020 - Jonathan Majors is confirmed as Kang for five MCU projects including Quantumania]

1st appearance and origin of Electro

[December 2021 - a source informs us that there was a clause in Foxx’s contract that secured his reprisal of the Electro role for a standalone feature if No Way Home hit an opening weekend monetary goal but we cannot say for sure that an Electro spin-off will happen]

1st appearance and origin of the Juggernaut

Origin of Professor X

1st cameo appearance of Cyttorak, as a statue, the demonic elder god whose Crimson Bands and ruby gemstone gave Juggernaut great powers

1st appearance of the Sentinels, machines invented to hunt mutants

1st appearance of Dr. Bolivar Trask, creator of the Sentinels

1st full appearance and cover of Sabretooth, a mutant who becomes the primary nemesis of Wolverine

1st appearance of the Green Goblin

First battle of Spider-Man versus The Hulk

1st appearance of Kraven the Hunter

2nd appearance of the Chameleon

First mention of Mary Jane Watson

[June 2021 - a Kraven movie is in-development with Aaron Taylor-Johnson in the lead role]

1st full appearance of Mystique, revealed without disguise

[April 2021 - when keying in a number sequence on the Buccaneer Bay section of Marvel’s exploremadripoor.com, an image with the name Mystique appears in a boat charter reservation]

1st appearance and origin of Scorpion, Mac Gargan appears in The Amazing Spider-Man #19

1st appearance of Rhino

1st appearance of Silver Surfer

1st cameo appearance of Galactus

[October 2021 - this is a rumor that comes from a solid source who has made it clear that it is not confirmed - the Silver Surfer is who James Gunn is referring to when teasing in a tweet about the Guardians 'will be introducing one of the greatest MCU characters of all time']

1st full appearance of Galactus

1st cover and 2nd appearance of the Silver Surfer

1st appearance of Mandarin

1st full appearance of Ultron

3rd appearance of Dane Whitman as the Black Knight

1st appearance of Thanos, an Eternal from Titan born with the Deviants gene

1st cameo appearance of Eros aka Starfox, brother of Thanos

1st appearance of Kronos, Grandfather of Thanos

1st appearance of Mentor, father of Thanos

1st appearance of Drax the Destroyer, created by Kronus to kill Thanos

1st appearance of the Blood Brothers

1st appearance of Loki, the Norse god of mischief

1st appearance of Balder, half-brother of Thor

1st appearance of Heimdall, guardian of Bifrost

1st appearance of Tyr, the Norse god of war

1st cameo appearance of Odin, the All-Father, seen from behind

3rd appearance of Thor

1st appearance of Fin Fang Foom, translated his name means 'he whose limbs shatter mountains and whose back scrapes the sun'

1st appearance of Dr. Chan Liuchow, a member of the Legion of Night

1st appearance and origin of Abomination, Emil Blonsky, one of the Hulk's arch-rivals

1st appearance of Byrrah in the Silver Age

1st appearance of M.O.D.O.K., a Mechanized Organism Designed Only for Killing

[January 2022 - Quantumania rumored to reintroduce a rebuilt Yellowjacket as M.O.D.O.K. / SDCC 2022 - M.O.D.O.K. confirmed to appear in Quantumania]

1st appearance of Morbius the Living Vampire

Debut of new powers, Jean Grey is reborn as the Phoenix

Ten years later this moment would be further developed where the Phoenix is an actual cosmic entity of vast power that copied Grey’s physical form while placing her body in a cocoon to heal from her injuries

1st full appearance of Black Tom Cassidy

1st appearance of Hela

1st and only appearance of Lady Sif, a different character than the Sif that appears in Thor #136

1st appearance of Enchantress, Amora

1st appearance of Skurge the Executioner

First Magneto appearance outside of an X-Men comic

1st appearance of Knorda, Queen of the Mountain Giants

2nd appearance of Doctor Strange

1st appearance of Baron Mordo, an evil apprentice of the Ancient One

1st appearance of Asbestos Man

1st appearance and origin of Absorbing Man

1st appearance of Fenris Wolf

1st appearance of Idunn, an Asgardian goddess

The Dark Phoenix Saga Part 1

1st appearance of Kitty Pryde

1st appearance of Emma Frost, the White Queen

1st cameo team appearance of The Hellfire Club, in shadows

1st appearance and origin of Bullseye

1st full appearance of the Taskmaster

[September 2022 - confirmed for the Thunderbolts movie]

1st full appearance of Mr. Sinister

[July 2022 - Javier Bardem rumored to be cast as Mr. Sinister in an unknown MCU project]

1st appearance of the Hobgoblin, Roderick Kingsley

A complete issue includes the ‘Tattooz' giveaway insert, found between the final page and the back cover. See additional images - be aware that the insert may be there but the tattoos might still be missing

[Feb 2021 - a social media account associated with Sony seemingly teased the transformation of Ned Leeds into the Hobgoblin - view image by tapping cover and swiping / June 2022 - Jacob Batalon reshared a photo holding a Hobgoblin action figure which caused future movie speculation on social media]

1st appearance and origin of Typhoid Mary

1st appearance of Nebula

[September 2021 - Gunn claims the roles of Nebula and Mantis are considerable in Guardians of the Galaxy Vol. 3]

1st full appearance of Venom

1st full appearance of Carnage, one of a race of extraterrestrial parasites known as the Symbiotes

Carnage is the offspring of Venom

[Carnage made his on-screen debut in Venom 2: Let There Be Carnage, played by Woody Harrelson]